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KMID : 0380620110430010119
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 1 p.119 ~ p.123
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Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods
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Chon Jung-Whan
Hyeon Ji-Yeon
Hwang In-Gyun
Kwak Hyo-Sun
Han Jeong-A
Kim Moo-Sang
Kim Jong-Hyun
Song-Kwang-Young
Seo Kun-Ho
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Abstract
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In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 mL) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.
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KEYWORD
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Campylobacter jejuni, real-time PCR, culture method, selective media
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